S-Adenosylhomocysteine (SAH) ELISA Kit

Cat.#:

JL58092

Regulatory Status:

RUO

Alternative:

S-Adenosyl-L-homocysteine;S-(5′-Deoxyadenos-5′-yl)-L-homocysteine;AdoHcy;2-S-adenosyl-L-homocysteine;5′-S-(3-Amino-3-carboxypropyl)-5′-thioadenosine S-adenosylhomocysteine

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

1.56-100pg/mL

Sensitivity:

0.87pg/mL

Specificity:

This kit recognizes SAH in samples. No significant cross-reactivity or interference between SAH and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cardiovascular Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SAH

S-Adenosyl-L-homocysteine (SAH) is the biosynthetic precursor to homocysteine.SAH is formed by the demethylation of S-adenosyl-L-methionine.Adenosylhomocysteinase converts SAH into homocysteine and adenosine.DNA methyltransferases are inhibited by SAH.Two S-adenosyl-L-homocysteine cofactor products can bind the active site of DNA methyltransferase 3B and prevent the DNA duplex from binding to the active site, which inhibits DNA methylation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	0.17	0.3	0.48	0.72	1.05	1.42	1.91	2.26

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of SAH spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	88-102	97
Plasma(n=8)	94-106	104
Cell Culture Supernates(n=8)	95-110	105

| Linearity

Samples were spiked with high concentrations of SAH and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-94	88-96	92-110
1 : 2	Average (%)	91	95	97
1 : 4	Range (%)	89-104	88-110	102-115
1 : 4	Average (%)	94	98	107